TABLE 1.

Affinity-purified human anti-gM/gN antibodies neutralize infectious HCMV AD169

Antibody	% Reduction in infectivity at an antibody dilution ofa:
	1:2	1:10	1:50
Anti-gBb	100 (100)	96 (94)	45 (39)
Anti-gM/gNb	99 (94)	40 (58)	0 (0)
Anti-gN MAbc (14-16a)	55 (100)	29 (99)	NT
Human IgGc	NT	100 (100)	100 (100)

^aInfectivity reduction of HCMV AD169 in a microtiter neutralization assay (1). The percentages of reduction in input infectivity are shown as the percentages of reduction in the absence (values not shown in parentheses) or presence (values in parentheses) of 5% guinea pig serum as a source of complement. Dilutions were made in Dulbecco's modified Eagle medium containing 10% fetal calf serum. NT, not tested.

^bAffinity-purified anti-gB and anti-gM/gN human antibodies were prepared as described in Materials and Methods at concentrations of approximately 1 μg/ml (anti-gB) and 0.5 μg/ml (anti-gM/gN).

^cThe anti-gN mouse MAb 14-16A and the pooled human IgG containing anti-HCMV antibodies were used as positive controls in these neutralization assays (5).