Figure 3.

Molecular analyses of the ninaB mutations. For sequence analyses β-diox cDNAs were cloned from homozygous ninaB^P315, homozygous ninaB^360d, heterozygous, and wt flies by RT-PCR with primers spanning the whole coding region. The alterations found in the ninaB alleles compared with the wt allele are indicated by arrows. (A) The sequence analyses of the cDNA isolated from ninaB^P315 revealed three base-pair exchanges at positions 838, 1,411, and 1,430 with respect to the deduced start ATG compared with the cDNA isolated from wt flies (AJ276682). All three alterations led to an alteration of the deduced amino acid sequence. (B) In the ninaB^360d, a point mutation at position 122 (C to A) was found, resulting in a nonsense codon in the deduced amino acid sequence.