TABLE 1.
Primers used for quantitative RT-PCR
| Target gene | Primera | Nucleotide sequence | GenBank accession no. |
|---|---|---|---|
| OAS1 (human) | F | 5′-GCCCTGGGTCAGTTGACTGG-3′ | NM_016816 |
| R | 5′-TGAAGCAGGTGGAGAACTCGC-3′ | ||
| ISG56 (human) | F | 5′-CAGCAACCATGAGTACAAAT-3′ | NM_001548 |
| R | 5′-AAGTGACATCTCAATTGCTC-3′ | ||
| IFN-α (human) | F | 5′-AAATACAGCCCTTGTGCCTGG-3′ | NM_000605 |
| R | 5′-GGTGAGCTGGCATACGAATCA-3′ | ||
| IFN-β (human) | F | 5′-AAGGCCAAGGAGTACAGTC-3′ | NM_002176 |
| R | 5′-ATCTTCAGTTTCGGAGGTAA-3′ | ||
| IFN-λ1 (human) | F | 5′-CGCCTTGGAAGAGTCACTCA-3′ | NM_172140 |
| R | 5′-GAAGCCTCAGGTCCCAATTC-3′ | ||
| IFN-λ2/3 (human) | F | 5′-AGTTCCGGGCCTGTATCCAG-3′b | NM_172138 |
| 5′-GAGCCGGTACAGCCAATGGT-3′b | |||
| 5′-ACTGCAGCCACTCCC-3′c | |||
| 5′-CTCCAGAACCTTCAGCGTCAG-3′c | |||
| PKR (human) | F | 5′-ACACTCGTCTCTGAATCATC-3′ | NM_002759 |
| R | 5′GAGACCATTCATAAGCAACG-3′ | ||
| β-Actin (human) | F | 5′-TCATCACCATTGGCATGAG-3′ | X00351, J00074, and M10278 |
| R | 5′-AGCACTGTGTTGGCGTACAG-3′ | ||
| IRF-7 (murine) | F | 5′-CCCAGACTGCCTGTGTAGACG-3′ | NM_016850 |
| R | 5′-CCAGTCTCCAAACAGCACTCG-3′ | ||
| ISG15 (murine) | F | 5′-AGCAATGGCCTGGGACCTAAA-3′ | NM_015783 |
| R | 5′-AGCCGGCACACCAATCTT-3′ | ||
| β-Actin (murine) | F | 5′-GCTCCCCGGGCTGTATTCC-3′ | NM_007393 |
| R | 5′-CTCTCTTGCTCTGGGCCTCGT-3′ |
a
The primers are identified as forward (F) and reverse (R).
b
Used in experiment with A549 cells.
c
Used in experiments with HeLa, Raji, U937, and HepG2 cells.