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. 2006 May;80(9):4227–4241. doi: 10.1128/JVI.80.9.4227-4241.2006

FIG. 2.

FIG. 2.

IKKγ-Δ mRNA expression. (A) IKKγ RT-PCR assay. Ethidium bromide staining of RT-PCR products fractionated by PAGE. Top panel, RT-PCR assay of various cellular RNA with IKKγ exon 4 and 6 primers. Bottom panel, housekeeping mRNA controls amplified from same source, GAPDH and DNA β-polymerase (β-Pol) are indicated. Lane 1, A549 human alveolar carcinoma cells; lane 2, human erythroleukemia (HL-60 cells) expressing Bcr-Abl; lane 3, human hepatocarcinoma Hep3B; lane 4, K562 erythroleukemia; lane 5, HL-60; lane 6, HeLa S3. (B) IKKγ expression in normal human tissues. Ethidium bromide staining of RT-PCR products fractionated by PAGE. Top panel, PCR products using IKKγ primers as in Fig. 2B. Bottom panel, PCR products for GAPDH and β-Pol. Lane 1, breast; lane 2, cervix; lane 3, kidney; lane 4, liver; lane 5, testicle; lane 6, adrenal gland; lane 7, colon; lane 8, lung; lane 9, pancreas.