FIG. 6.

(A) CCL2 mRNA expression is increased in rVV-G-primed CD8-deficient mice. Levels of CCL2 mRNA were assessed by RNase protection assays in whole lungs of wild-type and CD8^−/− C57BL/6 mice primed with rVV-G and challenged 2 weeks later with RSV. Results are expressed as protected band intensity and are percentages of the intensity of the housekeeping genes L32 and GAPDH. Controls are mRNA levels after rVV scarification prior to RSV infection and 24 h after intranasal inoculation of a Hep-2 cell lysate. *, P < 0.05 for comparison of wild-type and CD8^−/− mice. (B) CCL2 protein levels in cell-free BAL fluids from wild-type and CD8^−/− RSV-infected C57BL/6 mice were assessed by ELISA. The limit of detection is 37.5 pg/ml. In both panels, each data point is the mean for four animals. Error bars, standard errors.