FIG. 4.
Type I IFN-independent development of anti-SeV adaptive immunity. (a) H-2b NP peptide tetramer staining of CD8+ T cells specific for SeV in wild-type Sv129 and type I IFN receptor-deficient mouse lungs 10 days after infection. (b) In vivo cytotoxicity 10 days postinfection. Lysis of splenocytes pulsed with SeV peptide and labeled with a high dose of CFSE or mock pulsed and labeled with a low dose of CFSE was evaluated by flow cytometry 18 h after infusion into the infected mice. Two different animals are shown. Results are representative of more than three independent experiments. ko, type I IFN receptor-deficient mice.