FIG. 2.

Identification of BHV-1 Us9 and characterization of BHV-1 Us9 expression by BHV-5Us9-1 (Us9-exchanged BHV-5). (A) Immunoprecipitation-immunoblotting analysis of BHV-1-, BHV-5 Us9-1-, and BHV-5-infected cell lysates were immunoprecipitated with goat anti-BHV-1 Us9 antibody. After SDS-PAGE of the immunoprecipitated samples, they were immunoblotted with rabbit anti BHV-1 Us9 antibody. (B) Autoradiograph showing phosphorylation of Us9 expressed by BHV-1-, BHV-5-, and BHV-5 Us9-1-infected MDBK cells. MDBK cells were infected with the viruses designated above for each lane and were labeled with ³²P for 10 h starting at 3 hpi (BHV-1, Us9-deleted BHV-1, and BHV-5 Us9-1) or at 6 hpi (BHV-5 and BHV-5 Us9 deleted). The lysates were immunoprecipitated with goat anti-Us9 antibody as before.