TABLE 1.

List of primers used for PCR reactions

Primer	Name	Sequence
BHV-1 Us9 deleted
Us9 upstream	Forward-P1	5′ GAGTTTTTCGACGAGAATTCCTTCTCGGCCAGCATCGAC 3′
	Reverse-P2	5′ GCCGCACGAGGTACCGTGTGAGCGCTCAATTTATATGCAG 3′
Us9 downstream	Forward-P3	5′ CCGCGAGTAAAGCTTGTCTAATTTTTTCCGCACGC 3′
	Reverse-P4	5′ CATCGACGTCAGGATCCTCTTCCGCTGCATCGCCA 3′
BHV-5 Us9 deleted
Us9 upstream	Forward-P5	5′ GGCTGCAGTCCGAATTCTTCGACGAAGCGC 3′
	Reverse-P6	5′ GCGGGGTACCGCGCCGCCGCCGAATTTATACG 3′
Us9 downstream	Forward-P7	5′ CGCGGGCGAAAGCTTCACGGAGGACTGCTTC 3′
	Reverse-P8	5′ GCGACGTGAGCGGATCCGACTCCAGCAGCGAG 3′
BHV-1 Us9 ORF	Forward-P9	5′ CGCGGGTACCGCTGCATATAAATGGAGCGCTCACAC 3′
	Reverse-P10	5′ GGCCTCAAGCTTGGGGGTCTCGGGGCCTCAGGGCCG 3′
GFPa	Forward-P11	5′ CGAACTGAGGTACCTACAGCGTGAGC 3′
	Reverse-P12	5′ CGCGTTAAGCTTCATTGATGAGTTTGG 3′

^aAmplified by using modified plasmid pEGFP-C1 (1).