TABLE 3.
Summary of viral spread in the brain after intranasal inoculation
| Virus | Presence and location of viral antigena
|
|||||||
|---|---|---|---|---|---|---|---|---|
| OB | AON | PIR | HIPPO | AMYG | CG | LC | DR | |
| BHV-5 Us9-1 | ++++ | ++++ | ++++b | +++ | ++++ | ++++b | + | + |
| BHV-5 WT | ++ | ++++ | ++++ | +++ | ++++ | +++ | + | + |
| BHV-5 Us9Δ | − | − | − | − | − | − | − | − |
a
Indicated as no labeling (−) or 1 to 25 (+), 30 to 150 (++), 160 to 500 (+++), 550 to 3,000 (++++) labeled neurons per field at a magnification 5×. OB, olfactory bulb; AON, anterior olfactory nucleus; PIR, piriform cortex; HIPPO, hippocampus; AMYG, amygdale; CG, cingulated cortex; LC, locus coeruleus; DR, dorsal raphe. Scores for the BHV-5 Us9-1 are average for six rabbits; for each brain segment, five sections (total, 60 sections) were analyzed. Scores for BHV-5 WT and Us9-deleted virus are average for three rabbits, and for each segment, five sections were counted (15 slides for each section).
b
Upper end of the scale.