FIG. 2.

Plaque formation and replication kinetics of recombinant viruses. (A) Neutral red and X-Gal staining of plaques of recombinant MeVs on Vero/hSLAM cells. (B) Plaques and EGFP- and β-galactosidase-expressing individual cells (satellites are indicated by arrows) produced by 3 seg-MeV. (C) Replication kinetics. Vero/hSLAM cells were infected with viruses at a multiplicity of infection of 0.01. At various intervals, cells were harvested with culture medium, and the number of PFU was determined for Vero/hSLAM cells. (D) Production of satellites and plaques by 3 seg-MeV. The number of satellites (green bars) in the samples shown in panel C was counted and compared to the number of plaques (yellow bars).