FIG. 4.

Multiple foreign gene insertion into the 3 seg-MeV genome. (A) Diagram of the 3 seg-MeV-CAT genome. (B) Diagram of the 3 seg-MeV-CAT-SEAP genome. (C) Replication kinetics. Vero/hSLAM cells were infected with IC323-EGFP, 3 seg-MeV, 3 seg-MeV-CAT, and 3 seg-MeV-CAT-SEAP at a multiplicity of infection of 0.005. At various intervals, cells were harvested with culture medium, and the number of PFU was determined with Vero/hSLAM cells. (D and E) Analysis of CAT and SEAP enzymatic activities. In parallel to the results of PFU analysis shown in panel C, CAT activity in cells (D) and SEAP activity in culture medium (E) were quantified as described in Materials and Methods. SEAP activity is expressed in chemiluminescent units per second.