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. 2006 May;80(9):4510–4520. doi: 10.1128/JVI.80.9.4510-4520.2006

FIG. 5.

FIG. 5.

Interaction of HCV NS5B with CyPB in the JFH1 replicon. (A) Coimmunoprecipitation of endogenous CyPB with NS5B. Lysates from MH14#W31 (NN/1b/SG), JFH1#4-1 (JFH1/2a/SG), and Huh-7 cells as a negative control were used for immunoprecipitation with normal mouse immunoglobulin G (IgG) or anti-NS5B antibody (NS5B), followed by immunoblot analysis with either anti-CyPB (top) or anti-NS5B antibodies (bottom). IP, antibodies used for immunoprecipitation. (B) The interaction of CyPB with NS5B in JFH1 replicon was disrupted by CsA treatment. Coimmunoprecipitation between CyPB and NS5B was analyzed with MH14#W31 (NN/1b/SG) or JFH1#4-1 (JFH1/2a/SG) cells treated without CsA (lanes 1 and 5) or with CsA (0.3 μg/ml in lanes 2 and 6, 1 μg/ml in lanes 3 and 7, and 3 μg/ml in lanes 4 and 8).