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. 2006 May;80(9):4510–4520. doi: 10.1128/JVI.80.9.4510-4520.2006

FIG. 6.

FIG. 6.

CyPB in HCV replication of genotype 1b and JFH1. (A) Expression level of endogenous CyPB protein (top) and tubulin as an internal control (bottom) in MH14#W31 (NN/1b/SG), SN1 (Con1/1b/SG), sO (O/1b/SG), JFH1#4-1 (JFH1/2a/SG), and Huh-7 cells. (B) Knockdown of endogenous CyP proteins. MH14#W31 (NN/1b/SG) and JFH1#4-1 (JFH1/2a/SG) cells were transfected with siRNA specific for CyPA (si-CyPA), CyPB (si-CyP), a broad range of CyP subtypes [si-CyP(broad)], or a randomized siRNA (si-control). At 72 h posttransfection, CyPA (top), CyPB (middle) and tubulin as an internal control (bottom) were detected in total cell lysates of MH14#W31 (NN/1b/SG) (left) and JFH1#4-1 (JFH1/2a/SG) (right) cells by immunoblot analysis. (C) Depletion of CyPB did not affect HCV replication of JFH1 replicon. At 5 days posttransfection, HCV RNA titers in MH14#W31 (NN/1b/SG) (left) and JFH1#4-1 (JFH1/2a/SG) (right) cells were quantified by real-time RT-PCR analysis. no treatment, treatment with only the transfection reagent in the absence of siRNA. (D) Effect of siRNA on cell proliferation. Cell numbers of MH14W#31 (NN/1b/SG) and JFH1#4-1 (JFH1/2a/SG) cells treated with siRNA for 5 days were counted. Relative cell numbers were indicated.