TABLE 1.

Protection against EDIM shedding after i.n. immunization with VP6/LT(R192G) in neonatal and adult BALB/c mice challenged at 10, 28, or 42 dpi

Groupa	Time of challenge (dpi)	n	Quantity of antigen shed (ng/mouse/day)b	% Protectionc
Mock-immunized neonates		8	2,424 ± 4,843
VP6-immunized neonates	10	8	7,166 ± 17,497d,f	0
Mock-immunized neonates		7	1,817 ± 2,414
VP6-immunized neonates	28	8	426 ± 2,074e	76.6
Mock-immunized neonates		9	4,072 ± 5,399
VP6-immunized neonates	42	7	63 ± 224e	98.5
Mock-immunized adults		8	2,555 ± 3,820
VP6-immunized adults	10	8	290 ± 747e,g	88.6
Mock-immunized adults		8	4,384 ± 6,161
VP6-immunized adults	28	8	97 ± 262e	97.8
Mock-immunized adults		8	3,063 ± 4,370
VP6-immunized adults	42	7	51 ± 137e	98.3

^aNeonates were immunized at 7 days of age, and adults were immunized at ≥6 weeks of age.

^bMean quantity of rotavirus antigen shed ± standard deviation (SD) over 7 days postchallenge (ng/mouse/day).

^cPercent protection represents the percent decrease in mean quantity of rotavirus antigen shed during the 7 days after challenge compared to that for mock-immunized mice.

^dSignificantly (P = 0.007) greater than mock-immunized mice.

^eSignificantly (P ≤ 0.003) less than mock-immunized mice.

^fSignificantly (P ≤ 0.001) greater than immunized neonatal mice challenged at 28 or 42 dpi or immunized adult mice challenged at 10, 28, or 42 dpi.

^gSignificantly (P ≤ 0.04) greater than immunized adult mice challenged at 28 or 42 dpi.