TABLE 6.
Quantities of Th1, Th2, and ThIL-17 cytokines released from spleen cells obtained from mock- or vaccine-immunized mice after in vitro stimulationa
| Age at immunization | Immunization protocol | Time (dpi) | Quantity (pg/106 cells) of cytokine
|
|||
|---|---|---|---|---|---|---|
| IL-2 | IFN-γ | IL-5 | IL-17 | |||
| Neonate | Mockb | 10 | <1 | 101 | <1 | <1 |
| VP6/LT(R192G)b | 10 | <1 | 159 | <1 | <1 | |
| RRVc | 10 | 2 | 892 | <1 | <1 | |
| Mockb | 28 | <1 | 101 | <1 | <1 | |
| VP6/LT(R192G)b | 28 | 26 | 3,204 | 2 | 36 | |
| RRVc | 28 | 6 | 1,540 | <1 | 2 | |
| Adult | Mockb | 10 | <1 | 431 | <1 | 5 |
| VP6/LT(R192G)b | 10 | 79 | 5,139 | <1 | 220 | |
| RRVc | 10 | 3 | 990 | <1 | 2 | |
| Mockb | 28 | <1 | <1 | <1 | <1 | |
| VP6/LT(R192G)b | 28 | 98 | 12,629 | 121 | 121 | |
| RRVc | 28 | <1 | 8,004 | <1 | <1 | |
a
Quantities of cytokines represent differences between in vitro-stimulated and unstimulated cultures.
b
Cultures were stimulated with 4 μg of EDIM VP6::6XHis.
c
Cultures were stimulated with 10 μg of purified RRV particles.