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Eukaryotic chaperones disassemble papillomavirus (BPV) and polyomavirus (Py) virions and VLPs. In vitro reactions were performed by overnight dialysis against disassembly buffer. (A) TEM of samples with Hsc70 and Hsp40 chaperones (+hsc70/40). (B) Quantitation of fractions analyzed by sucrose gradient sedimentation.
