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. 2006 May;80(10):4820–4832. doi: 10.1128/JVI.80.10.4820-4832.2006

FIG. 3.

FIG. 3.

Detection of rotavirus antigen in lungs, hearts, thymuses, spleens, macrophages in lungs, and livers and colocalization of rotavirus antigen and macrophages in PBS- or RRV-inoculated rat pups. Immunoperoxidase detection of rotavirus antigen by anti-NSP4 antibody was done for lung sections of (A) RRV- or (B) PBS-inoculated rat pups at 96 hpi (arrows indicate morphologically identified macrophages), heart sections from (C) RRV- (arrow indicates stained cells) or (D) PBS-inoculated rat pups at 96 hpi, thymus sections from (E) RRV- or (F) PBS-inoculated rat pups at 96 hpi, spleen sections from (G) RRV- or (H) PBS-inoculated rat pups at 96 hpi, and (I) a macrophage in a blood vessel in a section of thymus from an RRV-inoculated rat pup at 96 hpi (arrow). Immunoperoxidase detection of macrophages by anti-CD68 staining was done for lung sections from (J) RRV- or (K) PBS-inoculated rat pups at 96 hpi (arrows indicate macrophages) and liver sections from (L) RRV- or (M) PBS-inoculated rat pups at 96 hpi. (N) Immunofluorescence detection of NSP4 and the macrophage marker CD68 in a lung section from an RRV-inoculated rat pup at 96 hpi, pseudocolored for NSP4 in green, for macrophages in red, and for nuclei in blue. Colocalization of NSP4 and the macrophage marker is shown in yellow. Arrows show pneumocytes infected with RRV, as detected by NSP4 staining. Magnification: A and B, ×1,000; J and K, ×300; C, D, I, and N, ×500; E, F, G, and H, ×400; L and M, ×10.