FIG. 5.

Kinetics of rotavirus-specific antigen detection by ELISA and infectious virus detection by FFA in PBS- or HAL1166-inoculated rat pups. (A) Serum; (B) liver. Each diamond represents an individual rat pup in each group, and each solid horizontal line represents the average OD value of each group at the indicated time point. The numbers above each column indicate the number of ELISA positive samples over the number of samples tested. The negative cutoff value for each organ, represented by the horizontal dotted line on each graph and the indicated numeric value, was determined by calculating 3 standard deviations above the mean of the OD values of the PBS control samples. The infectivities of samples were determined either by direct FFA or by passage of the sample on MA104 cells prior to FFA. Detection (circle around the diamond) or no detection (box around the diamond) of infectious virus is indicated for each sample tested.