TABLE 2.
Phenotypic coreceptor tropism assignment from prescreening of multiple clones derived from plasma virus
| Sample | No. of clones analyzed | No. (%) of clones with phenotypic tropism:
|
||
|---|---|---|---|---|
| CCR5 tropic | CXCR4 tropica | Dualtropic | ||
| Patient A | ||||
| Day 1b | 97 | 95 (98) | 0 | 2 (2) |
| Day 11 | 68 | 20 (29) | 0 | 48 (71) |
| Day 40 | 91 | 90 (99) | 0 | 1 (1) |
| Patient B | ||||
| Screen | 67 | 67 (100) | 0 | 0 |
| Day 1b | 118 | 118 (100) | 0 | 0 |
| Day 11 | 52 | 31 (60) | 0 | 21 (40) |
| Day 40 | 63 | 46 (73) | 0 | 17 (27) |
| Day 203 | 46 | 31 (67) | 2b (6) | 13 (28) |
| Day 251 | 44 | 30 (68) | 1c (2) | 13 (29) |
| Day 308 | 49 | 32 (65) | 4d (12) | 13 (22) |
| Day 373 | 48 | 41 (85) | 2e (4) | 5 (10) |
| Day 433 | 52 | 34 (65) | 16 (31) | 2 (4) |
| Patient C | ||||
| Day 1b | 25 | 14 (56) | 2f (8) | 9 (36) |
| Day 11 | 15 | 1 (7) | 5g (33) | 9 (60) |
| Day 40 | 26 | 23 (88) | 2h (8) | 1 (4) |
a
The screening assay was less sensitive than the full PhenoSense HIV Entry assay and had a tendency to score dualtropic clones as CXCR4-tropic. The following numbers indicate where the clone was tested in the PhenoSense HIV Entry assay for confirmation of CXCR4 tropism: b, 1/2 confirmed; c, 1/1 confirmed; d, 1/4 confirmed; e, 2/2 confirmed; f, 2/2 confirmed; g, 3/5 confirmed; h, 1/2 confirmed.
b
Day 1 samples were taken before treatment (predose).