TABLE 5.
In vitro activities of d4T, ddC, ddI, ABC, and TNV against HIV-1 encoding K65R and/or TAMs in RT
| HIV-1LAI mutation(s) in RT | Mean IC50 ± SD (fold change)a
|
||||
|---|---|---|---|---|---|
| d4T | ddC | ddI | ABC | TNV | |
| Wild type | 5.68 ± 3.28 | 1.35 ± 0.70 | 2.29 ± 0.97 | 6.33 ± 1.68 | 4.38 ± 2.02 |
| TAM41 | 6.74 ± 5.91 (1.2) | 1.28 ± 0.70 (0.9) | 1.91 ± 0.90 (0.8) | 7.22 ± 4.96 (1.1) | 3.98 ± 1.24 (0.9) |
| TAM67 | 13.1 ± 7.4 (2.3)b | 1.19 ± 0.53 (0.9) | 2.75 ± 1.60 (1.2) | 12.8 ± 4.1 (2.0)b | 3.55 ± 1.27 (0.8) |
| K65R | 21.8 ± 23.8 (3.8) | 6.79 ± 3.98 (5.0) | 6.26 ± 2.92 (2.7) | 26.3 ± 4.2 (4.2) | 12.1 ± 4.3 (2.8) |
| TAM41/65Rc | 8.44 ± 5.15 (1.5) | 2.90 ± 1.21 (2.2)d | 4.78 ± 2.91 (2.1) | 16.3 ± 4.2 (2.6)e | 10.7 ± 6.9 (2.4) |
| TAM67/65Rc | 11.8 ± 4.8 (2.1) | 2.81 ± 1.15 (2.1)d | 4.23 ± 2.48 (1.8) | 15.1 ± 6.3 (2.4)e | 4.52 ± 0.77 (1.0)e |
a
IC50 values were determined by measuring inhibition of luminescence in P4/R5 cells. Values are means ± standard deviations from at least three independent experiments. Fold change is relative to the wild type.
b
IC50 is significantly different from the wild type (P < 0.01).
c
Antagonism or trend in antagonism is indicated in bold.
d
P = 0.04 (compared to HIV-165R).
e
IC50 is significantly different from HIV-165R (P < 0.005).