FIG. 2.
Effect of a matrix mutation (L30E) on envelope function. (A) 293T cells were transfected with pSVIIIenv (NL4.3 env) with either env− pNL4.3 or env− pNL4.3 (L30E) and assessed for envelope expression (left panels) and cell-cell fusion (right panels). (B) Impact of L30E mutation in p17gag on virus infectivity tested on GHOST/CXCR4 cells and represented as infectivity (FFU) to particle (RT) ratio. (C) Envelope incorporation into virus particles was assessed by measuring the amounts of envelope in transfected 293T cell supernatants. Virus samples were precipitated and resuspended in PBS, and equivalent amounts of RT activity were then resolved on a sodium dodecyl sulfate-8% polyacrylamide gel, followed by Western blotting. Envelope and p24 were probed with Chessie 8 and 183-H12-5C monoclonal antibodies, respectively.