FIG. 4.

Envelope association with lipid rafts in MOLT 4 T cells. MOLT 4 cells were infected with VSV-G⁺ pseudotype virions prepared with either pNL4.3 wt or pNL4.3 (L30E). After 48 h, cells were lysed with cold 0.5% Triton X-100 and homogenized, and postnuclear supernatants were subjected to sucrose density gradient centrifugation as described in Materials and Methods. Gradient fractions were subsequently probed for envelope (gp160 and gp41) and Gag (p55) with mouse MAbs by Western blotting. Note that envelope proteins coexpressed with the L30E Gag mutation failed to associate with DRM-L (bottom panel) compared with that with wild-type Gag (top panel).