FIG. 2.
CVB infections in islets of 12-week-old, prediabetic NOD mice colocalize with different islet cell types. A dose of 5 × 105 TCID50 CVB3/28 was inoculated into mice; mice were killed 4 days later, and serial pancreas sections were stained for insulin (A), somatostatin (E), pancreatic polypeptide (I), and glucagon (M) or virus capsid protein (B, F, J, and N). Merged images (C, G, K, and O) show examples of virus protein colocalized with the specific cell marker protein (arrows) at an original magnification of ×480. Hematoxylin and eosin-stained sections (D, H, L, and P) show the extent of islet inflammation at an original magnification of ×480 (D, L, and P) or ×240 (H).