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Increased ORF50 mRNA in BAC36ΔLNA. BAC36ΔLNA (Δ) or BAC36wt (wt) was transfected into 293 cells and assayed by conventional RT-PCR (A) or real-time PCR (B) at 24, 48, or 72 h posttransfection. A) RT-PCR was measured for ORF50/Rta, ORF73/LANA, and cellular GAPDH. B) Real-time PCR analysis of cDNA products was performed for ORF50 mRNA relative to BAC-encoded GFP mRNA.
