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. 2006 Jun;80(11):5273–5282. doi: 10.1128/JVI.02541-05

FIG. 5.

FIG. 5.

NaB-dependent acetyl lysine reactivity with LANA. A) 293 cells transfected with control or FLAG-LANA expression vectors were treated with or without 1 mM NaB for 6 h. Input FLAG-LANA protein was analyzed by Western blotting of starting extracts (lanes 1 to 4). Immunoprecipitates (IP) with control IgG (lanes 5 to 8) or anti-acetyl lysine antibody (lanes 9 to 12) were analyzed by Western blotting with anti-FLAG antibody. B) Same as described for panel A, except 293 cells were treated with 500 ng/ml TSA for 6 h. C) 293 cells were transfected essentially as described for panel A, immunoprecipitated with anti-FLAG antibody, and then assayed by Western blotting with antibody to acetyl lysine (left panel) or to anti-FLAG (right panel). D) BCBL1 or DG75 cells were treated with 1 mM NaB for 6 h (+) or left untreated (−) and then subjected to immunoprecipitation with anti-acetyl lysine antibody (middle panel) or control IgG (right panel). Immunoprecipitates were analyzed by Western blotting with anti-LANA-specific antibody. Input extract was analyzed in the right-hand panel as indicated. IB, immunoblot.