FIG. 1.

(A) Schematic of the control vector pNL-SIN-CMV-FL, which encodes FLuc, and the indicator vector pNL-SIN-CMV-RL, which encodes RLuc. In each RLuc-based indicator construct, two perfectly complementary target sequences for one KSHV miRNA were inserted into the 3′UTR of the Renilla luciferase gene. (B) BJAB, BC-1, and BCBL-1 cells were transduced with a mixture of the control and indicator viruses, and dual luciferase assays were performed 24 h later. RLuc activities were normalized to the observed FLuc activity. Normalized RLuc activities in KSHV-positive BC-1 and BCBL-1 cells are shown relative to those obtained in KSHV-negative BJAB cells. Values from cells transduced with the parental RLuc-expressing vector were set at 100%. LTR, long terminal repeat.