FIG. 4.

Ganglioside incorporation corrects the PPMP-induced inhibition of BAAV transduction. COS cells were pretreated with 5 μM PPMP before ganglioside-loaded liposomes were added to the cells. The cells were cultured in the presence of PPMP and liposomes before they were transduced with rBAAV-GFP or rAAV5-GFP. Twenty-four hours after transduction, the cells were analyzed for GFP expression by flow cytometry. The values are means from three experiments; the error bars represent standard deviations.