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Infectivity of the pB-AAV clone. 293 cells or MDBK cells in Lab-Tek two-chambers slides were infected with 2 μl of cell lysates from pB-AAV-transfected 293 cells, plus Ad5 at an MOI of 5 (panel B and D), or 2 μl of cell lysates from B-AAVRepCap (a nonreplicating plasmid)-transfected 293 cells, plus Ad5 at an MOI of 5 (panel A and C). Immunofluorescence assays were performed at 36 to 40 h postinfection, using an AAV anti-Rep monoclonal antibody that recognizes B-AAV Rep proteins.
