FIG. 8.

Rep transactivation of the B-AAV P41 promoter requires both adenovirus and the Rep-binding site. 293 cells were transfected, in the presence of Ad5 (MOI of 5), with the reporter plasmids diagrammed in panel B, with (+) or without (−) the Rep-supplying plasmid (B-AAVRepSM). Plasmid C1GFP (Clontech) was cotransfected as a control and was protected by a probe to the GFP coding region (40). The levels of P41 RNA (Unspl+Spl) were normalized to GFP RNA, and the activation fold (Act. Fold) is presented. The quantifications of the activation fold and the spl/unspl RNA ratio were averages with standard deviations of at least from two independent experiments. A partial sequence of the P7 promoter is diagrammed in panel B; the TATA box and the putative RBE-like sequence are indicated.