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Protease protection assay of VLPs containing VP35 and VP40. VLP pellets were split into six fractions and treated with STE buffer (lane 1), soybean trypsin inhibitor (lane 2), Triton X-100 (lane 3), trypsin (lane 4), trypsin and Triton X-100 (lane 5), and trypsin and soybean trypsin inhibitor (lane 6). The fractions were divided equally, and viral proteins were immunoprecipitated with α-VP40 (A) or α-HA (B). Samples were resolved by SDS-PAGE and detected by autoradiography.
