FIG. 2.

Adenoviral vectors expressing GFP from the CMV promoter, but not the UbC promoter, induce TAp73 expression. MEFs suspended in serum-free medium were transduced with adenovirus at the indicated MOI, plated at subconfluency for 16 h in complete medium, changed to starvation medium (0.2% serum) for 2 days to synchronize their cell cycles, and then fed for 22 h in complete medium prior to harvest. (A) The transcript levels of TAp73 relative to 18S rRNA were determined by real-time RT-PCR. Indistinguishable results were obtained by normalization to β-actin. Parallel samples were used for GFP analysis (gray diamonds). The standard error of the mean is included (obscured when the error is very small). (B) TAp73 protein was detected by immunoblotting. A nonspecific band served as the loading control (lower blots).