FIG. 3.

Adenoviral vectors expressing GFP from the CMV promoter, but not the UbC promoter, induce ΔNp73 expression. (A) MEFs were transduced with adenovirus at the indicated MOI, serum starved, and then restimulated with serum-containing medium as described in the legend to Fig. 2. The transcript levels of ΔNp73 relative to 18S rRNA were determined by real-time RT-PCR. Parallel samples were used for GFP analysis (gray diamonds). The standard error of the mean is included (obscured when the error is very small). (B) EL4 thymoma cells expressing the coxsackievirus/adenovirus receptor required for adenoviral entry (26) were transduced over a wide range of MOIs, and the percentage that expressed GFP was quantified via flow cytometry 16 h later.