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. 2006 Jun;80(11):5349–5360. doi: 10.1128/JVI.02016-05

FIG. 5.

FIG. 5.

CDK-pRb-E2F pathway is required for adenoviral induction of TAp73. (A) MEFs were transduced as before (MOI, 10), and TAp73, CycA2, DHFR, and Cdc6 RNAs relative to 18S rRNA were quantified by real-time RT-PCR. (B) MEFs were incubated in 30 μM roscovitine or dimethyl sulfoxide (DMSO) control, and CycA2, DHFR, and Cdc6 RNAs relative to 18S rRNA were quantified by real-time RT-PCR. (C) MEFs were transduced as before (MOI, 10), except the 0.2% starvation medium was not replaced or (D) roscovitine or DMSO control was added when the starvation medium was replaced with 10% FBS-DMEM. (E) MEFs were transduced with the indicated adenoviral vectors at the indicated MOIs. Additional Ad CMV-GFP was added to equalize the MOIs when Ad CMV-p16 was used. TAp73 mRNA (black bars, left y axis) and adenoviral pIX RNA (gray bars, right y axis) relative to 18S rRNA were quantified by real-time RT-PCR. The standard error of the mean is included (obscured when the error is very small).