FIG. 7.

Adenoviral vectors possessing only endogenous elements induce adenoviral pIX and TAp73 expression. MEFs were transduced as before with adenoviral vectors that maintain the natural E1A promoter but lack foreign promoter sequences. As indicated in Fig. 1, the two adenoviral vectors used differed in that only the first possessed an SV40 polyadenylation sequence between the E1A promoter and the pIX coding sequence. TAp73 mRNA (black bars, left y axis) and adenoviral pIX RNA (gray bars, right y axis) relative to 18S rRNA were quantified by real-time RT-PCR. “DEAD” indicates that cell killing at these MOIs prevented mRNA analyses. For both viruses, a linear correlation (correlation coefficient, 0.99) exists between pIX RNA and TAp73 induction as a function of the MOI used for transduction being decreased. The standard error of the mean is included (obscured when the error is very small).