FIG. 8.

Chemotherapeutics and Ad CMV-GFP cooperatively decrease cellularity. MEFs were transduced with adenovirus (MOI, 30) as before, except 0.2% FBS starvation medium was replaced with 10% FBS-DMEM containing DMSO, etoposide (10 μM), or 5-FU (100 μM). Four days following the addition of chemotherapeutics, triplicate wells were harvested and the quantity of live cells was assessed by forward and side scatter as described in Materials and Methods. The standard error of the mean is included. *, P < 0.005; **, P < 0.0005 using Student's two-sample homoscedastic t test.