FIG. 1.

Approximate location of the siRNA target sequences within an optimal structure of the HAV genome (strain 18f). Sequence M59808 was augmented with known 5′- and 3′-terminal bases and then analyzed by mFold. Base numbers (shown in blue) are in increments of 1,000. Each base in the minimum free energy structure is color coded on a sliding scale, according to its pairing number (P-num), with red, orange, yellow, green, blue, and violet indicating successively higher frequencies of potential alternative configurations within an energy window of +12 kcal from the optimal (44).