TABLE 1.

Overlapping 15-mer peptides synthesized for this study

HSV-2 ORF	ORF class	Common name	ORF length (aa)	No. of 15-mer peptides	Peptide overlap (aa)	No. of library pools	No. of peptides/library pool
UL54	IE	ICP27	512	126	11	2	63, 63
RL2	IE	ICP0	825	164	10	3	50, 50, 64
US1	IE	ICP22	413	81	10	2	41, 41
US12	IE	ICP47	86	16	10	1	16
RS1	IE	ICP4	1,318	161	10	5	50, 50, 50, 50, 62
UL6	Capsid		678	134	10	2	67, 67
UL15	Capsid		735	145	10	2	73, 74
UL18	Capsid	VP23	318	69	11	1	69
UL19	Capsid	VP5/ICP5	1,374	273	10	5	50, 50, 50, 50, 73
UL25	Capsid		585	115	10	2	50, 65
UL26/UL26.5	Capsid	VP21, VP24	625	123	10	2	61, 62
UL35	Capsid	VP26	113	21	10	1	21
UL38	Capsid	ICP32	467	92	10	1	92
UL4	Tegument		202	39	10	1	39
UL11	Tegument		97	18	10	1	18
UL13	Tegument		519	102	10	2	51, 51
UL14	Tegument		220	42	10	1	42
UL21	Tegument		530	104	10	2	50, 52
UL36	Tegument	ICP1-2	3,122	623	10	7	89, 89, 89, 89, 89, 89, 89
UL37	Tegument		1,114	221	10	4	50, 50, 50, 71
UL41	Tegument	VHS	492	97	10	2	50, 47
UL46	Tegument	VP11/12	722	146	10	3	50, 50, 46
UL47	Tegument	VP13/14	696	172	11	3	50, 50, 72
UL48	Tegument	VP16	490	96	10	2	48, 48
UL49	Tegument	VP22	300	73	11	1	73
UL51	Tegument		245	47	10	1	47
US3	Tegument		481	100	10 (11)a	2	33, 67
US9	Tegument		90	16	10	1	16
US10	Tegument		303	59	10	1	59
US11	Tegument		151	29	10	1	29
UL1	Glycoprotein	gL	225	43	10	1	43
UL10	Glycoprotein	gM	468	92	10	1	92
UL20	Membrane protein		223	43	10	1	43
UL22	Glycoprotein	gH	839	166	10	2	83, 83
UL27	Glycoprotein	gB	905	179	10	2	89, 90
UL44	Glycoprotein	gC	481	94	10	1	94
UL45	Membrane protein		173	33	10	1	33
UL53	Glycoprotein	gK	339	66	10	1	66
US4	Glycoprotein	gG	700	138	10	2	69, 69
US5	Glycoprotein	gJ	93	17	10	1	17
US6	Glycoprotein	gD	394	77	10	1	77
US7	Glycoprotein	gI	373	73	10	1	73
US8	Glycoprotein	gE	545	107	10	2	50, 57
UL23	Other		136	31	11	1	31
UL29	Other	ICP8	1,196	238	10	5	50, 50, 50, 50, 38
UL39	Other	ICP6	1,142	227	10	4	50, 50, 50, 77
UL50	Other	dUTPase	369	72	10	1	72
UL55	Other		187	36	10	1	36

^aThe N-terminal 33 peptides overlapped by 10 amino acids; the remainder overlapped by 11 amino acids.