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. 2006 Jun;80(11):5156–5167. doi: 10.1128/JVI.02349-05

FIG. 5.

FIG. 5.

EBOV infection inhibits IFN-β-induced expression of MxA and prevents nuclear translocation of PY-STAT1. (A) Mock-infected Vero cells express MxA in response to overnight treatment with IFN-β (lane 2). However, EBOV-infected cells (MOI = 1) do not produce MxA in response to IFN-β (lane 4). Lanes 1 and 2 were mock infected. Lanes 3 and 4 were infected with EBOV. Lanes 2 and 4 were treated with IFN-β, while lanes 1 and 3 were mock treated. (Top panel) Western blot to detect MxA and GAPDH. (Bottom panel) Western blot to detect VP24. (B) Mock-infected Vero cells translocate PY-STAT1 to the nucleus after 30 min of IFN-β treatment (top panel), but EBOV-infected cells retain PY-STAT1 in the cytoplasm (bottom panel). Red represents viral antigen (VP35), and green represents PY-STAT1.