FIGURE 4.
Cytolytic capacity of PBMC after multiple courses of g209-2M peptide vaccination. Pre- (left panels) and postimmunization course 4 PBMC (right panels) from patients 1-5 were rested overnight in CM and analyzed for cytolytic activity against T2 target cells alone (triangles) or pulsed with 1 μM native g209 (squares) or g280 (circles) in a 4-hour 51Cr-release assay. Values represent the mean percent lysis from triplicate wells [H11506] SEM at the indicated effector-to-target-cell ratio. Asterisk indicates a significant difference (P < 0.05) between g209-specific and g280 control lysis determined by two-sided Kruskal-Wallis test.