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. 2006 May;50(5):1731–1737. doi: 10.1128/AAC.50.5.1731-1737.2006

FIG. 1.

FIG. 1.

Allicin prevents cleavage of CSP. (A) P. berghei sporozoites were metabolically labeled with [35S]Cys/Met and kept on ice (labeled 0) or chased for 2 h in the absence of protease inhibitors (labeled 2), in the presence of 10 μM E-64 (labeled E-64), or in the presence of the indicated concentrations of allicin (labeled 10, 25, and 50). The lane labeled 50dil represents labeled sporozoites chased in the presence of 50 μM allicin for 10 min, which was then diluted to 4.2 μM for the remainder of the chase. After 2 h, the parasites were lysed and CSP was immunoprecipitated and analyzed by SDS-polyacrylamide gel electrophoresis and autoradiography. (B). P. berghei sporozoites were incubated in the presence or absence of 50 μM allicin for 10 min and then added to Hepa 1-6 cells for 2 min before being fixed and stained with antisera specific for full-length CSP. Two hundred sporozoites/well were counted, and means ± standard deviations for duplicate samples are shown.