Figure 4.

Hematopoietic and immune parameters in the absence of α-mannosidase II. (A) Serum Ig levels comprising IgM, IgA, and IgG were elevated by 10 weeks of age (16 mice of each genotype used). Elevations in IgG levels included IgG1, IgG2a, and IgG2b, whereas no changes were seen in IgE or IgG3 levels (not shown). (B) Lymphocyte cellularity was not altered among lymphoid organs or in circulation. Increased cellularity in the spleen was due to increased numbers of nucleated erythroblasts, as described (14). Frequencies of T cells (CD4⁺ and CD8⁺) and B cells (B220⁺) were also normal. (C) T and B cell proliferation after antigen receptor crosslinking was unaffected. At east five mice of each genotype were studied.