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. 2001 Jan 30;98(3):1142–1147. doi: 10.1073/pnas.98.3.1142

Figure 5.

Figure 5

Anti-nuclear antibodies and autoantibodies are found in mice lacking α-mannosidase II. (A) Reactivity of wild-type sera (wt) or α-mannosidase II deficient sera (Δ/Δ) to HEpG2 cells visualized by fluorescent microscopy (×200). Nucleolar (Bottom left) and nuclear membrane (Bottom right) binding by immunoglobulins in the sera of different mice. Sera dilutions were 1:250. (B) Autoantibody reactivity to various nuclear antigens. Eight mice of each genotype were tested (wild type plotted as OD ± SD, Δ/Δ plotted individually). (C) Autoantibodies to kidney, lung, and liver proteins are induced in α-mannosidase II-deficient mice. Similarly increased autoreactivity is noted when wild-type protein or α-mannosidase II-deficient protein is used as the substrate. (D) Cellular proteins of the indicated genotype were subjected to SDS-PAGE with or without PNGase F pretreatment. Protein blots were incubated with indicated sera (1:2,000 dilution) and processed as described (Materials and Methods). Identical genotypes represent autologous samples in C and D.