FIG. 3.

CAM at 10 μg/ml increases the proportion of NHBE cells in G₁ phase (P < 0.0001) and decreases the proportions in S phase (P < 0.01) and G₂/M phase (P < 0.001). Cells were seeded in 25-cm² flasks and cultured in the absence (top graph) or presence (bottom graph) of CAM. Growth factors were withdrawn from the culture medium 24 h before CAM exposure. At 48 h, flow cytometric analysis was performed. The area under the white curve is the raw data. The filled area is the curve fit for the G₁, S, and G₂/M phases of the cell cycle. The tall peak represents cells in G₁, and the crosshatched area represents cells in S phase from the DNA histogram. These experiments were repeated three times, and data are shown as means ± SEMs. Cont, control.