FIG. 2.
The plasma and mitochondrial membranes of P. falciparum generate a high transmembrane electrochemical potential (Ψm). Confocal laser scanning microscopy of live P. falciparum-infected erythrocytes loaded with the potentiometric probe TMRE. The panels show bright-field fluorescence (a) and fluorescence (b) images of an infected erythrocyte loaded with TMRE, TMRE fluorescence of parasite mitochondria from bafilomycin-treated parasites (c, d, and e), and TMRE fluorescence from merozoites (f). The green in these images is a pseudocolor. TMRE was excited at 543 nm, and emission was collected with a 560-nm long pass filter. Bars, 2 μm.