FIG. 4.
Effect of mitochondrial inhibitors on mitochondrial Ψm. Time course of TMRE-dependent fluorescence of P. falciparum-infected erythrocytes after the addition of rotenone (50 μM), atovaquone (10 μM), and FCCP (10 μM) (A) and SHAM (1 mM), bafilomycin (200 nM), and FCCP (10 μM) (B). Inhibitors were also tested against bafilomycin-treated cells. Time-dependent TMRE fluorescence was monitored after addition of rotenone (50 μM), atovaquone (10 μM), and FCCP (10 μM) (C) and flavone (0.5 mM) and FCCP (10 μM) (D). Data were normalized to 100% in untreated (A and B) or bafilomycin-treated (C and D) cells and to 0% in FCCP (10 μM)-treated cells. Graphs show means from experiments performed independently ± standard errors (n ≥ 6).