FIG. 5.
Effect of DPI on P. falciparum mitochondrial Ψm. TMRE-dependent fluorescence was monitored with time after the addition of DPI (10 μM) to untreated (A) and bafilomycin-treated (B) malaria-infected erythrocytes. Data represent means from independent experiments ± standard errors (n ≥ 9). (C) Concentration dependence of DPI on the initial rate of mitochondrial Ψm depolarization. Data points are means from three individual experiments. Data were fitted by nonlinear regression analysis (Marquart method) using an iterative procedure to generate the best fit (χ2) of the curve to the data. Standard errors were calculated for each parameter using the matrix inversion method (Grafit Software).