Figure 3.

Effects of misexpression of SOC1, FT, and FLC in different genetic backgrounds on flowering time. (A) Flowering times are expressed as total leaf numbers of soc1-1 transgenic plants in which SOC1 is expressed from tissue-specific promoters. All plants were grown under LDs. (B) As A, but plants were grown under SDs. (C) Flowering times of transgenic plants misexpressing FLC and either FT or SOC1. Plants were grown under LDs. Some genotypes involve crosses of Columbia FRI flc-3 to Ler, but because the Ler FLC allele does not respond to FRI, no activation of endogenous FLC occurs (Michaels and Amasino 1999). (D) Flowering times of transgenic lines expressing SOC1 from tissue-specific promoters in co-2 or soc1-1 and carrying the ft-7 mutation, as well as double mutants carrying ft-7 and other flowering-time mutations. Plants were grown under LDs. (E) Analysis of FT mRNA abundance by RT–PCR in the leaves of Ler, co-2, SUC2∷SOC1 co-2, and SUC2∷CO co-2 grown under SDs. Leaves were harvested at dawn.