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. 2006 Apr 15;20(8):1004–1014. doi: 10.1101/gad.1408106

Figure 4.

Figure 4.

Effects of overexpression of NRK1-nonphosphorylatable NtMAP65-1a on the response to propyzamide of cortical and phragmoplast MTs. (A) Localization of GFP-NtMAP65-1a (MAP65:WT) and GFP-NtMAP65-1a:T579A (MAP65:T579A) in transformed BY-2 cells. Cells were incubated for 12 h with 0.5 μM DEX and then incubated with or without 3 μM propyzamide for another 12 h. (B) Distribution of MTs in BY-2 cells that expressed GFP-NtMAP65-1a (MAP65:WT), GFP-NtMAP65-1a:T579A (MAP65:T579A), GFP-NtMAP65-1a:T501A;S503A (MAP65:T501A;S503A), and GFP-NtMAP65-1a:T501A;S503A;T579A (MAP65:T501A;S503A;T579A) in the absence and the presence of propyzamide. Cells were incubated as noted above and then double-stained with mouse antibodies against tubulin (green) and DAPI (for nuclei; red). Bottom panels show images of tubulin (arrows) only to emphasize the stability of MT arrays in cells that expressed the NRK1-nonphosphorylatable protein. (N) Nucleus.