Auto-Abs in BALB/c mice immunized with the 16/6Id. BALB/c mice
(five mice per group) were immunized with 16/6 Id and concomitantly
injected with pCDR1 or reversed pCDR1(100 μg/mouse i.v. once per
week for 5 weeks) or were not treated. Results expressed as OD ±
SD were obtained at the bleeding before sacrifice and represent all
monthly bleedings. (A) Anti-DNA Ab titers measured on
individual sera (dilution 1:1,000). (B) Anti-16/6Id Ab
titers measured on individual sera (dilution 1:10,000).
(C) Abs to nuclear antigens measured on pooled sera
(dilution 1:100). OD levels of normal sera and of sera of mice that
were injected only with pCDR1 were undetectable. The results are
representatives of three experiments. (A) *,
P < 0.05 as compared with reversed pCDR1-treated
mice. †, P < 0.01 as compared with 16/6Id
immunized untreated mice. (B) $, P
< 0.05 as compared with 16/6Id immunized untreated or *
16/6Id immunized and reversed pCDR1-treated mice.