Skip to main content
. 2006 Apr 15;20(8):939–953. doi: 10.1101/gad.1388706

Figure 4.

Figure 4.

Derepression activity of TDE correlates with the UV-cross-linking of 38-kDa protein. (A) The luciferase activity of the Fluc constructs containing 5′ Her-2 UTR and internal and 3′-end deletions of Her-2 3′ UTR. (B) The 3′ Her-2 UTR UV-cross-linked to a 38-kDa protein from SKBR-3 extracts. Proteins UV-cross-linked to labeled RNA probes were resolved on 10%–14.5% SDS-PAGE as described in Materials and Methods. Molecular size markers are indicated on the right. (C) Competition of the UV-cross-linked band by 73-nt TDE but not by the sequences of the 5′ UTR of Her-2 mRNA. The T7 polymerase-transcribed 32P-labeled 3′ Her-2 UTR RNA was incubated with SKBR-3 cytoplasmic extracts in the absence of competitor (none) or in the presence of a 2.5-fold, fivefold, 20-fold, 50-fold, or 100-fold molar excess of unlabeled 465–537-nt RNA or with a 10-fold, 50-fold, 100-fold, or 500-fold molar excess of the 178-nt 5′ Her-2 UTR RNA.